Active pharmaceutical ingredients and mechanisms underlying phasic myometrial contractions stimulated with the saponin extract from Paris polyphylla Sm. var. yunnanensis used for abnormal uterine bleeding

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Active pharmaceutical ingredients and mechanisms underlying phasic myometrial contractions stimulated with the saponin extract from Paris polyphylla Sm. var. yunnanensis used for abnormal uterine bleeding.

BACKGROUND Total steroidal saponins of Paris polyphylla Sm. var. yunnanensis (TSSP) have been widely used in China for the treatment of abnormal uterine bleeding (AUB). But until now, the main active constituents and the mechanisms underlying the pharmacological actions on uterine activity have not been described. METHODS Total steroidal saponins were extracted with EtOH and purified by chrom...

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Intracellular mechanisms underlying prostaglandin F2α-stimulated phasic myometrial contractions.

These studies sought to test the hypothesis that prostaglandin F2α(PGF2α)-stimulated phasic myometrial contractions are characterized by the activation of the phosphatidylinositol-signaling pathway resulting in the generation of cytosolic calcium oscillations. For the experiments described in this report rat myometrial tissue was used, after the tissue was loaded with fura 2, to perform cytosol...

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Intracellular mechanisms underlying prostaglandin F2a-stimulated phasic myometrial contractions

Phillippe, Mark, Trevania Saunders, and Andrew Basa. Intracellular mechanisms underlying prostaglandin F2a-stimulated phasic myometrial contractions. Am. J. Physiol. 273 (Endocrinol. Metab. 36): E665–E673, 1997.—These studies sought to test the hypothesis that prostaglandin F2a (PGF2a)-stimulated phasic myometrial contractions are characterized by the activation of the phosphatidylinositolsigna...

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Pennogenin Tetraglycoside Induces Rat Myometrial Contraction and MLC20 Phosphorylation via PLC-IP3 and RhoA/Rho Kinase Signaling Pathways

BACKGROUND Total steroidal saponins extracted from the rhizome of Paris polyphylla Sm. var. yunnanensis (TSSPs) have been widely used in China for the treatment of abnormal uterine bleeding. We previously studied the main active constituents of TSSPs and their structure-activity relationships with respect to rat myometrial contractions. Tg (pennogenin tetraglycoside) was identified as one of th...

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In-vitro Antitumor Activity and Antifungal Activity of Pennogenin Steroidal Saponins from paris Polyphylla var. yunnanensis

Paris polyphylla Smith var. yunnanensis, has been used in traditional Chinese medicine for its antibiotic and anti-inflammatory properties; in addition it has been used to cure liver cancer in particular. In this current study, β-ecdysterone (1) and three pennogenin steroidal saponins (2-4) were isolated from the EtOH extract of Paris polyphylla var. yunnanensis, and then tested for their antit...

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ژورنال

عنوان ژورنال: Human Reproduction

سال: 2008

ISSN: 0268-1161,1460-2350

DOI: 10.1093/humrep/den001